Journal: Nutrients

Article Title: Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction

doi: 10.3390/nu14061213

Figure Lengend Snippet: Effects of propolis on the expressions of the LKB1/AMPK signaling pathway in the jejunal tissue determined. ( A ) Western blotting analysis of the expression level of RhoA, AMPK, p-AMPK, LKB1 and p-LKB1 in jejunal tissues. ( B ) Quantitative analysis of the western blotting data. * Denotes p < 0.05 vs. control group; △ p < 0.05 vs. model group.

Article Snippet: Subsequently, the membranes transferred with protein were blocked using a Tris-buffered saline (TBS) buffer containing 5% BSA for 30 min at room temperature, and then incubated with specific primary antibodies, including anti-Ras homolog gene family member A (RhoA) (1:1000), Claudin-1 (1:1000), Occludin (1:1000), ZO-1 (1:1000), liver kinase B1 (LKB1) (1:1000), p-LKB1 (1:1000), AMP-activated protein kinase (AMPK) (1:1000), p-AMPK (1:1000) and β-actin (1:1000) (Cell Signaling Technology, Danvers, MA, USA), overnight at 4 °C, respectively. β-actin was chosen as the reference for internal standardization.

Techniques: Western Blot, Expressing, Control

Journal: Nutrients

Article Title: Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction

doi: 10.3390/nu14061213

Figure Lengend Snippet: Effects of propolis on the expressions of LKB1/AMPK signaling pathway in the colon tissue. ( A ) Western blotting analysis of the expression level of RhoA, AMPK, p-AMPK, LKB1 and p-LKB1 in colon tissues. ( B ) Quantitative analysis of the western blotting data. * Denotes p < 0.05 vs. control group; △ p < 0.05 vs. model group.

Article Snippet: Subsequently, the membranes transferred with protein were blocked using a Tris-buffered saline (TBS) buffer containing 5% BSA for 30 min at room temperature, and then incubated with specific primary antibodies, including anti-Ras homolog gene family member A (RhoA) (1:1000), Claudin-1 (1:1000), Occludin (1:1000), ZO-1 (1:1000), liver kinase B1 (LKB1) (1:1000), p-LKB1 (1:1000), AMP-activated protein kinase (AMPK) (1:1000), p-AMPK (1:1000) and β-actin (1:1000) (Cell Signaling Technology, Danvers, MA, USA), overnight at 4 °C, respectively. β-actin was chosen as the reference for internal standardization.

Techniques: Western Blot, Expressing, Control